案例五: 熒光測量
2019/08/07
Sample Setup: Fluorescence
Overview
Fluorescence measurements require a sensitive detector and an effective filter for discriminating between powerful excitation source wavelengths and weak spectral emissions from the sample.
Spectrometer
We offer several spectrometers useful for fluorescence, but recommend the high-sensitivity, preconfigured USB4000-FL Spectrometer for most general fluorescence applications. The USB4000-FL is set to 360-1000 nm and comes with a 200-m slit and an L4 Detector Collection Lens for increased light throughput.
Sampling Optics
Your standard excitation source option is our PX-2 Pulsed Xenon Source. Our proprietary LVF Linear Variable Filters are excellent tools for spectrally shaping the excitation energy from broadband sources used for fluorescence. Various sampling optics are available for detecting picomolar-range concentrations of fluorophores from surfaces and in solutions and powders.